A phthalimidation protocol that follows protein defined parameters.
نویسندگان
چکیده
This work outlines the first phthalimidation protocol suitable for protein labeling and performed in aqueous media at room temperature and neutral pH with no catalyst or co-reagent required. The methodology is suitable for a range of amines and its efficiency was determined with chemoselective and site-selective protein labeling.
منابع مشابه
P-45: Predictive Value of Follicular Fluid VitaminD on Assisted Reproductive TechniqueOutcome
Background: Vitamin D, a steroid hormone, has critical roles in human health. The available data thus identify vitamin D as an important substance in processes involved in reproductive success and thereby suggest pathophysiological mechanisms for reproductive compromise in the setting of vitamin D deficiency. Realizing the prevalence of vitamin D insufficiency in our country, we hypothesized th...
متن کاملComparing the Bidirectional Baum-Welch Algorithm and the Baum-Welch Algorithm on Regular Lattice
A profile hidden Markov model (PHMM) is widely used in assigning protein sequences to protein families. In this model, the hidden states only depend on the previous hidden state and observations are independent given hidden states. In other words, in the PHMM, only the information of the left side of a hidden state is considered. However, it makes sense that considering the information of the b...
متن کاملبهینه سازی روش شستشوی غشاهای لبنی
The Tagouchi method was used for providing the instruction of ultra-filter dairy membrane cleaning. The goal function with using of four undimentionalized parameter, i.e., water and milk serum flux, percent of protein and dry mass of permeate, as quality indicator of cleaning of cleaned membrane, was defined. As a result, basic cleaning solution was found as follows: 0.8% NaOH, 1.0% EDTA, 1.0% ...
متن کاملO-9: The Peroxidation Indices during Vitrification of Bovine Ovary
Background: Cryopreservation of ovary for preserving germ cell line is going to be an important issue. However, the physical and chemical stresses during cryopreservation are the most important factors that may influence the post freezing tissue viability and quality. Vitrification is a new approach to bypass the impacts of both stresses for gametes and embryos and it is also suggested for ovar...
متن کاملA New Two Step Induction Protocol for Neural Differentiation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells
Background: In this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. Materials and Methods: Human umbilical cord blood-derived mesenchymal stem cells were first cultured in Dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 51 3 شماره
صفحات -
تاریخ انتشار 2015